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|[ Article ]|
|New & Renewable Energy|
|Abbreviation: New. Renew. Energy|
|ISSN: 1738-3935 (Print) 2713-9999 (Online)|
|Online publication date 19 Aug 2021|
|Received 11 May 2021 Revised 26 Jul 2021 Accepted 04 Aug 2021|
|합성가스 발효에서 배지 내 Vitamin 농도의 에탄올 생산에 대한 영향|
임홍래1), 4) ; 안태광1), 4) ; 박소은2) ; 김영기3), *
Effect of Culture Medium Vitamin Concentration of Culture Medium on Ethanol Production in Syngas Fermentation
Hongrae Im1), 4) ; Taegwang An1), 4) ; Soeun Park2) ; Young-Kee Kim3), *
|1)Master Candidate, Faculty of Food Biotechnology and Chemical Engineering, Hankyong National University|
|2)Researcher, Research Center of Chemical Technology, Hankyong National University|
|3)Professor, Faculty of Food Biotechnology and Chemical Engineering, Hankyong National University|
|4)These authors contributed equally to this work|
|Correspondence to : *email@example.com Tel: +82-31-670-5206|
© 2021 by the New & Renewable Energy
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this study, we assessed the effect of vitamin components (such as biotin, thiamine-HCl, and folic acid) on microorganism microbial growth and ethanol production was examined to enhance increase the ethanol concentration in the Clostridium autoethanogenum culture process using syngas as a sole carbon source. Biotin and folic acid concentrations of 0.2, 2, 20, and 100 μg/L were used in the culture experiments at 0.2, 2, 20, and 100 μg/L concentrations. The maximum ethanol concentrations of 2.81 g/L and 3.12 g/L were obtained by adding at 0.2 μg/L biotin and folic acid, respectively. Moreover, Thiaminethiamine-–HCl at concentrations of 0.5, 5, 50, and 250 μg/L were was examined evaluated to in the culture experiments. The maximum ethanol concentration of 2.84 g/L was observed at 0.5 μg/L of thiamine-–HCl. As a resultThus, the optimized concentrations of biotin, thiamine-–HCl, and folic acid were determined at 0.2, 0.5, and 0.2 μg/L, respectively, for enhancing increasing the ethanol production. In conclusion, the maximum ethanol production was obtained by adding the minimal concentration of vitamins in C. autoethanogenum culture.
|Keywords: Clostridium autoethanogenum, Syngas fermentation, Biotin, Thiamine-HCl, Folic acid, Medium optimization
키워드: 클로트리디움 오토에타노게놈, 합성가스 발효, 비오틴, 티아민, 엽산, 배지 최적화
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