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New. Renew. Energy

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New & Renewable Energy
Abbreviation: New. Renew. Energy
ISSN: 1738-3935 (Print) 2713-9999 (Online)
Online publication date 19 Aug 2021
Received 11 May 2021 Revised 26 Jul 2021 Accepted 04 Aug 2021

합성가스 발효에서 배지 내 Vitamin 농도의 에탄올 생산에 대한 영향
임홍래1), 4) ; 안태광1), 4) ; 박소은2) ; 김영기3), *

Effect of Culture Medium Vitamin Concentration of Culture Medium on Ethanol Production in Syngas Fermentation
Hongrae Im1), 4) ; Taegwang An1), 4) ; Soeun Park2) ; Young-Kee Kim3), *
1)Master Candidate, Faculty of Food Biotechnology and Chemical Engineering, Hankyong National University
2)Researcher, Research Center of Chemical Technology, Hankyong National University
3)Professor, Faculty of Food Biotechnology and Chemical Engineering, Hankyong National University
4)These authors contributed equally to this work
Correspondence to : * Tel: +82-31-670-5206

© 2021 by the New & Renewable Energy
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this study, we assessed the effect of vitamin components (such as biotin, thiamine-HCl, and folic acid) on microorganism microbial growth and ethanol production was examined to enhance increase the ethanol concentration in the Clostridium autoethanogenum culture process using syngas as a sole carbon source. Biotin and folic acid concentrations of 0.2, 2, 20, and 100 μg/L were used in the culture experiments at 0.2, 2, 20, and 100 μg/L concentrations. The maximum ethanol concentrations of 2.81 g/L and 3.12 g/L were obtained by adding at 0.2 μg/L biotin and folic acid, respectively. Moreover, Thiaminethiamine-–HCl at concentrations of 0.5, 5, 50, and 250 μg/L were was examined evaluated to in the culture experiments. The maximum ethanol concentration of 2.84 g/L was observed at 0.5 μg/L of thiamine-–HCl. As a resultThus, the optimized concentrations of biotin, thiamine-–HCl, and folic acid were determined at 0.2, 0.5, and 0.2 μg/L, respectively, for enhancing increasing the ethanol production. In conclusion, the maximum ethanol production was obtained by adding the minimal concentration of vitamins in C. autoethanogenum culture.

Keywords: Clostridium autoethanogenum, Syngas fermentation, Biotin, Thiamine-HCl, Folic acid, Medium optimization
키워드: 클로트리디움 오토에타노게놈, 합성가스 발효, 비오틴, 티아민, 엽산, 배지 최적화